HiDef® PBS/EDTA Cell Dissociation Reagent Size:1 x 125 mLDefined Biosciences HiDef PBS EDTA is a defined reagent for protein free adherent cell dissociation, for routine aggregate passaging or single cell suspension. HiDef PBS EDTA is designed to detach cells from surfaces without impacting cell functionality or surface protein integrity, with applications in stem, tumor, and primary cell culture, among others. HiDef PBS EDTA can also be used as a cell washing step prior to detachment using other methods,
Defined Bioscience’s HiDef® PBS/EDTA is a defined reagent for protein-free adherent cell dissociation
Each lot of HiDef® S8 400X Supplement is used in combination with basal medium during performance qualification using multiple human PSC lines cultured under suspension conditions in both ambient and hypoxic environments
although optional surfactants such as polyvinyl alcohol (PVA)
The HiDef® B8 400X Supplement only (basal medium NOT included)
with benefits in cell passaging
00 mg/mL insulin
and related additives may be used depending on the application and bioreactor configuration
HiDef® S8 is currently provided as:
transferrin (to facilitate iron transport)
HiDef® B8 400X Supplement compatibility has also been verified with several manufacturers’ formulations of DMEM/F12 such as Corning® (#10-092)
FGF2-G3 (human) (FGF2-STAB) is a stabilized growth factor that offers a novel way to grow FGF2-dependent cell cultures more efficiently
and depending on your intended use
